SHANK3 anticorps (AA 840-857)

N° du produit ABIN492466

Détail du produit anti-SHANK3 anticorps

Antigène: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Épitope: AA 840-857

Reactivité: Rat, Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SHANK3 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Detects ~190 kDa protein. No cross-reactivity against Shank1 or Shank2.

Attributs du produit: Synonyms: PROSAP2, PSAP2, SPANK-2, SPANK2, KIAA1650, SH3 and multiple ankyrin repeat domainsprotein 3, Proline-rich synapse-associated protein 2

Purification: Protein G Chromatography

Immunogène: Synthetic peptide amino acids 840-857 of Rat Shank3 (accession number Q9JLU4). AA Sequence: PEKLPGSLRKGIPRTKSV

Clone: S69-46

Isotype: IgG2b

Information d'application

Indications d'application: Western blot: 1 μg/mL1 μg/mL was sufficient for detection of Shank3 in 10 μg of COS cell (lysate) transientlytransfected with Shank3 by colorimetric immunoblot analysis using Goat anti-mouseIgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur SHANK3

Antigène: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Autre désignation: SHANK3 (SHANK3 Produits)

Synonymes: anticorps DEL22q13.3, anticorps PROSAP2, anticorps PSAP2, anticorps SCZD15, anticorps SPANK-2, anticorps Prosap2, anticorps AI841104, anticorps Shank3b, anticorps SH3 and multiple ankyrin repeat domains 3, anticorps SH3 and multiple ankyrin repeat domains protein 3, anticorps SHANK3, anticorps shank3, anticorps Shank3

Sujet: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (3). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are also crucial in receptor tyrosine kinase signaling, specifically, Shank3 can mediate Erk-MAPK and P13K signaling which is crucial for tubule formation (4). Shank3 is also one of the latest genes to be associated with autism. A mutation of a single copy of Shank3 on chromosome 22q13 can result in language and/or social communication disorders (5).Synonyms: KIAA1650, PROSAP2, PSAP2, Proline-rich synapse-associated protein 2, SH3 and multiple ankyrin repeat domains protein 3, SPANK-2, SPANK2

ID gène: 59312

UniProt: Q9JLU4

Pathways: Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity