TRPC5 anticorps (Trp5)

N° du produit ABIN492474

Détail du produit anti-TRPC5 anticorps

Antigène: TRPC5 (Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5))

Épitope: AA 827-845, Trp5

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TRPC5 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Detects ~110 kDa protein.

Attributs du produit: Synonyms: TRP-5, Short transient receptor potential channel 5

Purification: Protein G Chromatography.

Immunogène: Synthetic peptide amino acids 827-845 of Human TrpC5.

Clone: S67-15

Isotype: IgG2b

Information d'application

Indications d'application: Western blot: 1 μg/mL (if results are poor, use lysate without boiling, heat at 37 °C for 15 minutes).1 μg/mL was sufficient for detection of Kv3.4 in 10 μg of Rat brain lysate by colorimetricimmunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur TRPC5

Antigène: TRPC5 (Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5))

Autre désignation: TRPC5 (TRPC5 Produits)

Synonymes: anticorps trpc5, anticorps wu:fi73f08, anticorps TRPC5, anticorps Trpc5, anticorps TRP5, anticorps CCE2, anticorps TRP-5, anticorps Trrp5, anticorps trp, anticorps transient receptor potential cation channel, subfamily C, member 5a, anticorps transient receptor potential cation channel subfamily C member 5, anticorps transient receptor potential cation channel, subfamily C, member 5, anticorps trpc5a, anticorps TRPC5, anticorps Trpc5

Sujet: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Transient receptor potential cation channel, subfamily C, member 5, also known as TRPC5, is a subtype of the TRPC family of mammalian transient receptor potential ion channels. Homomultimeric TRPC5 and heteromultimeric TRPC5-TRPC1 channels are activated by extracellular reduced thioredoxin (3). This activation probably plays a role in rheumatoid arthritis. It has also been recently found to be involved in the action on anaesthetics such as choloform, halothane and propofol (4).Synonyms: Short transient receptor potential channel 5, TRP-5, Transient receptor protein 5

ID gène: 7224

UniProt: Q9UL62