Recombinant Lapin anti-Humain IgG4 Anticorps
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- Antigène Tous les produits IgG4
- IgG4
- Type d'anticorp
- Recombinant Antibody
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Monoclonal
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)
- Attributs du produit
- This recombinant Human IgG4 antibody reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
- Purification
- Protein A purified from animal origin-free supernatant
- Immunogène
- Peptide corresponding to the hinge region of human IgG4 was used as the immunogen for this recombinant Human IgG4 antibody.
- Clone
- RM120
- Isotype
- IgG
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- Indications d'application
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The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. ELISA: 50 ng/well - 200 ng/well (Capture), 0.05-0.2 μg/mL (Detection),Western Blot: 0.5-2 μg/mL,Immunocytochemistry: 1-10 μg/mL,Immunohistochemistry: 1-10 μg/mL (1)
- Restrictions
- For Research Use only
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- Buffer
- 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store the recombinant Human IgG4 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
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- Antigène
- IgG4
- Abstract
- IgG4 Produits
- Classe de substances
- Antibody
- Sujet
- This recombinant Human IgG4 antibody reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
- ID gène
- 3503
- UniProt
- P01861
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