p53 anticorps (AA 364-393)

N° du produit ABIN3029395

Cet anticorps Lapin Polyclonal détecte spécifiquement p53 dans WB, IHC et ELISA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour p53 anticorps (AA 364-393) (ABIN3029395)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-p53 anticorps

Épitope: AA 364-393

Fonction: p53 Antibody / TP53

Purification: Purified

Immunogène: A portion of amino acids 364-393 from the human protein was used as the immunogen for this anti-p53 antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: Titration of the anti-p53 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles

Stock: -20 °C

Stockage commentaire: Aliquot the anti-p53 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53

Sujet: Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. P53 is subject to modification by conjugation of SUMO-1. A p53 mutant deficient for MDM2 binding is poorly sumoylated in vivo compared to wild-type p53. Overexpression of MDM2 increases the level of p53 sumoylation, which is further stimulated by expression of ARF. These results show that p53 sumoylation is regulated by MDM2- and ARF-mediated nucleolar targeting.

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg