p53 anticorps (pSer315)

N° du produit ABIN3029388

Cet anticorps anti-p53 est un anticorps Lapin Polyclonal détectant p53 dans IHC, ELISA et DB. Adapté pour Humain.

Aperçu rapide pour p53 anticorps (pSer315) (ABIN3029388)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Immunohistochemistry (IHC), ELISA, Dot Blot (DB)

Détail du produit anti-p53 anticorps

Épitope: pSer315

Réactivité croisée (Details): Expected species reactivity: Primate

Purification: Antigen affinity purified

Immunogène: This phospho-p53 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS315 of human p53.

Isotype: Ig Fraction

Information d'application

Indications d'application: Titration of the phospho-p53 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the phospho-p53 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53

Sujet: Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg