EIF2AK2 anticorps (pThr451)

N° du produit ABIN498761

L’anticorps anti-EIF2AK2 Polyclonal Lapin est utilisé pour la détection de EIF2AK2 dans des échantillons de Humain. Il a été validé pour IHC (p).

Aperçu rapide pour EIF2AK2 anticorps (pThr451) (ABIN498761)

Antigène: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF2AK2 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-EIF2AK2 anticorps

Épitope: pThr451

Specificité: This antibody detects endogenous levels of PKR protein only when phosphorylated at Thr451.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur EIF2AK2

Antigène: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Autre désignation: EIF2AK2 / PKR

Sujet: An interferon-inducible, RNA-dependent protein Serine/Threonine kinase, PKR has various designations. Mouse PKR is known as DAI, dsJ, PI kinase, p65, p67 or TIK, whereas human PKR is known as p68 or p69. PKR phosphorylates its substrate, a subunit of protein synthesis initiation factor eIF-2, on Ser 51 to inhibit translation. PKR contains two dsRNA binding motifs required for its activation by dsRNA. Three kinds of regulation of PKR enzymatic activity occur, and these include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation, and post-translational regulation by RNA mediated autophosphorylation. Human PKR contains at least 15 autophosphorylation sites, but only Thr 446 and Thr 451 in the activation loop are critical for its kinase activity. Thr 446 is the in vivo autophosphorylation site of PKR.Synonyms: Eukaryotic translation initiation factor 2-alpha kinase 2, Interferon-induced double-stranded RNA-activated protein kinase, PRKR, Protein kinase RNA-activated, eIF-2A protein kinase 2, p68 kinase

Poids moléculaire: Predicted band size: 62 kDaObserved band size: 68 kDa

ID gène: 5610

NCBI Accession: NP_001129123

UniProt: P19525

Pathways: Réparation de l'ADN, ER-Nucleus Signaling, Hepatitis C