Western Blotting (WB), ELISA, Immunofluorescence (IF)
Fonction
Rabbit polyclonal antibody raised against synthetic phosphopeptide of AURKB.
Specificité
Reactivity occurs against human Aurora Kinase B pT232 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A.
Réactivité croisée
Chimpanzé, Boeuf (Vache), Chien, Humain, Souris, Porc, Rat
Attributs du produit
Antibody Reactive Against Synthetic Peptide.
Immunogène
Synthetic phosphopeptide corresponding to residues surrounding T232 of human AURKB.
AURKB
Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc
WB, IHC, IHC (p)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA (1:10000-1:50000) Western Blot (1:250-1:2000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Yasui, Urano, Kawajiri, Nagata, Tatsuka, Saya, Furukawa, Takahashi, Izawa, Inagaki: "Autophosphorylation of a newly identified site of Aurora-B is indispensable for cytokinesis." dans: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12997-3003, (2004) (PubMed).
Kimura, Uchida, Takano, Kitagawa, Okano: "Cell cycle-dependent regulation of the human aurora B promoter." dans: Biochemical and biophysical research communications, Vol. 316, Issue 3, pp. 930-6, (2004) (PubMed).
Gruneberg, Neef, Honda, Nigg, Barr: "Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2." dans: The Journal of cell biology, Vol. 166, Issue 2, pp. 167-72, (2004) (PubMed).