Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Fonction
Rabbit polyclonal antibody raised against synthetic peptide of SMAD3.
Specificité
ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated human Smad3. Expect reactivity against Smad1 and Smad5. Negligible reactivity is seen against other Smad family members.
SMAD3
Reactivité: Humain, Souris, Rat
WB, IHC (p), FACS, IF (cc)
Hôte: Lapin
Monoclonal
3D1
unconjugated
Indications d'application
ELISA (1:10000-1:40000) Western Blot (1:500-1:3000) Immunohistochemistry (1:500-1:3000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Shi, Massagué: "Mechanisms of TGF-beta signaling from cell membrane to the nucleus." dans: Cell, Vol. 113, Issue 6, pp. 685-700, (2003) (PubMed).
Derynck, Zhang: "Smad-dependent and Smad-independent pathways in TGF-beta family signalling." dans: Nature, Vol. 425, Issue 6958, pp. 577-84, (2003) (PubMed).
Antigène
SMAD3
(SMAD, Mothers Against DPP Homolog 3 (SMAD3))