SMAD3 anticorps (pSer423, pSer425)

N° du produit ABIN539529

Détail du produit anti-SMAD3 anticorps

Antigène: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Épitope: pSer423, pSer425

Reactivité: Humain, Souris, Rat, Porc, Poulet, Poisson zèbre (Danio rerio), Boeuf (Vache), Grenouille

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD3.

Specificité: Reactivity occurs against human Smad3 pS423 and pS425 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members.

Réactivité croisée: Poulet, Boeuf (Vache), Grenouille, Humain, Souris, Porc, Rat, Poisson zèbre (Danio rerio)

Attributs du produit: Antibody Reactive Against Synthetic Peptide.

Immunogène: Synthetic phosphopeptide corresponding to residues surrounding S423/S425 of human SMAD3.

Information d'application

Indications d'application: ELISA (1:75000-1:300000)
Western Blot (1:2000-1:20000)
Immunohistochemistry (1:500)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Références pour anticorps anti-SMAD3 (ABIN539529)

Zhang, Jiang, Tian, Wang, Zhao, Wang, Li, Liu, Li, Zhang, Guan: "CB2R orchestrates fibrogenesis through regulation of inflammatory response during the repair of skeletal muscle contusion." dans: International journal of clinical and experimental pathology, Vol. 8, Issue 4, pp. 3491-502, (2015) (PubMed).

Yamashita, Fatyol, Jin, Wang, Liu, Zhang: "TRAF6 mediates Smad-independent activation of JNK and p38 by TGF-beta." dans: Molecular cell, Vol. 31, Issue 6, pp. 918-24, (2008) (PubMed).

Shi, Massagué: "Mechanisms of TGF-beta signaling from cell membrane to the nucleus." dans: Cell, Vol. 113, Issue 6, pp. 685-700, (2003) (PubMed).

Derynck, Zhang: "Smad-dependent and Smad-independent pathways in TGF-beta family signalling." dans: Nature, Vol. 425, Issue 6958, pp. 577-84, (2003) (PubMed).

Détails sur SMAD3

Antigène: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Autre désignation: SMAD3 (SMAD3 Produits)

Synonymes: anticorps HSPC193, anticorps HsT17436, anticorps JV15-2, anticorps LDS1C, anticorps LDS3, anticorps MADH3, anticorps madh3, anticorps madh3a, anticorps smad3, anticorps wu:fa99e03, anticorps XSmad3, anticorps XenMLP, anticorps Xmad3, anticorps madh3-A, anticorps Madh3, anticorps AU022421, anticorps Smad 3, anticorps mad3, anticorps madh3b, anticorps zgc:92234, anticorps SMAD family member 3, anticorps SMAD family member 3a, anticorps SMAD family member 3 L homeolog, anticorps SMAD family member 3b, anticorps SMAD3, anticorps smad3a, anticorps smad3.L, anticorps Smad3, anticorps smad3b

ID gène: 4088

Pathways: Cycle Cellulaire, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy