Pyruvate Kinase anticorps (HRP)

N° du produit ABIN5596779

Détail du produit anti-Pyruvate Kinase anticorps (HRP)

Antigène: Pyruvate Kinase (PK)

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Pyruvate Kinase est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): This product has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other tissues and species may occur but have not been specifically determined.

Purification: Pyruvate Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle].

Immunogène:

Immunogen: Pyruvate Kinase [Rabbit Muscle]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Pyruvate Kinase has been assayed against 1.0 μg of Pyruvate Kinase [Rabbit Muscle] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Pyruvate Kinase

Antigène: Pyruvate Kinase (PK)

Autre désignation: Pyruvate Kinase (PK Produits)

Synonymes: anticorps CG7070, anticorps Dmel\\CG7070, anticorps PYK, anticorps Pyk, anticorps PYK3b, anticorps PYK3c, anticorps Pyruvate kinase, anticorps PK, anticorps pyruvate kinase, anticorps pyruvate kinase (PK), anticorps Pyruvate kinase (PK), anticorps pyruvate kinase PKM, anticorps testis expressed 45, anticorps PyK, anticorps pykA, anticorps PYK3a|PYK3b|PYK3c, anticorps pyk, anticorps pyk1, anticorps pk, anticorps LOC100534543, anticorps TEX45

Sujet:

Synonyms: Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, HRP

Background: Pyruvate Kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Genetic defects of this enzyme cause the disease known as pyruvate kinase deficiency and can cause hemolytic anemia.

Gene Name: PKM2

ID gène: 100008676

UniProt: P11974