CDKN1B anticorps (Internal Region, pSer140)
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- Antigène Voir toutes CDKN1B Anticorps
- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
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Épitope
- Internal Region, pSer140
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp CDKN1B est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Réactivité croisée (Details)
- This antibody will specifically react with a p27kip1 protein from human tissue. Reactivity against p27 from other species is unknown.
- Purification
- Anti-p27 kip1 pS140 is directed against the phosphorylated form of human p27 kip1 protein at the S140 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.
- Immunogène
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Immunogen: Anti-p27 kip1 pS140 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to S140 residue of human p27 kip1 protein.
Immunogen Type: Peptide
- Isotype
- IgG
- Top Product
- Discover our top product CDKN1B Anticorps primaire
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- Indications d'application
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-p27kip1 pS140 antibody is suitable for use in ELISA, immunoprecipitation, co-immunoprecipitation and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~27 kDa in size corresponding to p27 by western blotting in the appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human p27. Optimal titers for other applications should be determined by the researcher.
Western Blot Dilution: 1:500 - 1:4,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:10,000 - 1:40,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.68 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Date de péremption
- 12 months
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Upregulation of human glycolipid transfer protein (GLTP) induces necroptosis in colon carcinoma cells." dans: Biochimica et biophysica acta. Molecular and cell biology of lipids, Vol. 1864, Issue 2, pp. 158-167, (2019) (PubMed).
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Upregulation of human glycolipid transfer protein (GLTP) induces necroptosis in colon carcinoma cells." dans: Biochimica et biophysica acta. Molecular and cell biology of lipids, Vol. 1864, Issue 2, pp. 158-167, (2019) (PubMed).
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- Antigène
- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
- Autre désignation
- p27 kip1 (CDKN1B Produits)
- Synonymes
- anticorps CDKN4, anticorps KIP1, anticorps MEN1B, anticorps MEN4, anticorps P27KIP1, anticorps cdkn1b, anticorps sb:cb611, anticorps wu:fb63g10, anticorps wu:fb64g10, anticorps wu:fe18e03, anticorps CDKN1B, anticorps Kip1, anticorps p27, anticorps FAM14D, anticorps ISG12, anticorps ISG12A, anticorps P27, anticorps Cdki1b, anticorps AA408329, anticorps AI843786, anticorps p27Kip1, anticorps cdkn1bl, anticorps cyclin dependent kinase inhibitor 1B, anticorps cyclin-dependent kinase inhibitor 1Bb, anticorps interferon alpha inducible protein 27, anticorps cyclin-dependent kinase inhibitor 1B, anticorps cyclin-dependent kinase inhibitor 1Ba, anticorps CDKN1B, anticorps cdkn1bb, anticorps IFI27, anticorps Cdkn1b, anticorps cdkn1ba
- Sujet
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Synonyms: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1,
Background: p27 kip1 pS140 is a cyclin-dependent kinase inhibitor that shares a limited similarity with CDK inhibitor CDKN1A/p21. p27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Phosphorylation occurs on serine, threonine and tyrosine residues. Anti-p27 kip1 pS140 antibody is ideal for researchers interested in cell cycle and cancer research.
Gene Name: CDKN1B
- ID gène
- 1027
- UniProt
- P46527
- Pathways
- Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
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