PFKM anticorps (HRP)

N° du produit ABIN5596852

Détail du produit anti-PFKM anticorps (HRP)

Antigène: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PFKM est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity against Fructose-6-Phosphate Kinase from other sources may occur but have not been specifically determined.

Purification: Fructose-6-Phosphate Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle].

Immunogène:

Immunogen: Fructose-6-Phosphate Kinase [Rabbit Muscle]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Fructose-6-Phosphate Kinase antibody has been tested for use in ELISA, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur PFKM

Antigène: PFKM (phosphofructokinase, Muscle (PFKM))

Autre désignation: Fructose-6-Phosphate Kinase (PFKM Produits)

Synonymes: anticorps GSD7, anticorps PFK-1, anticorps PFK1, anticorps PFKA, anticorps PFKX, anticorps AI131669, anticorps PFK-A, anticorps PFK-M, anticorps Pfk-4, anticorps Pfk4, anticorps Pfka, anticorps Pfkx, anticorps Pfk-M, anticorps M-PFK, anticorps PFKM, anticorps pfkm, anticorps zgc:92344, anticorps pfk, anticorps im:7137110, anticorps wu:fc58g11, anticorps phosphofructokinase, muscle, anticorps phosphofructokinase, platelet, anticorps phosphofructokinase, muscle a, anticorps phosphofructokinase, muscle S homeolog, anticorps phosphofructokinase, muscle b, anticorps PFKM, anticorps Pfkm, anticorps PFKP, anticorps pfkma, anticorps pfkm.S, anticorps pfkmb

Sujet:

Synonyms: 6 Phosphofructokinase Muscle Type antibody, EC 2.7.1.11 antibody, GSD7 antibody, MGC8699 antibody, PFKA antibody, PFKL antibody, PFKM antibody, PFKP antibody, PFKX antibody, Phosphofructo 1 Kinase Isozyme A antibody, Phosphofructokinase 1 antibody, HRP

Background: Fructose-6-Phosphate Kinase -2 (F6PK) also known as Phosphofructokinase (PFK) catalyzes the conversion of ATP + D-fructose 6-phosphate to ADP + D-fructose 1,6-bisphosphate and therefore is a key enzyme in the control of glycolysis and carbohydrate degradation. This is a unidirectional and rate-limiting step in glycolysis. Allosteric kinetics control activation by ADP, AMP, or fructose bisphosphate and inhibition by ATP or citrate. The enzyme exists as a homotetramer.

Gene Name: PFKM

ID gène: 100345647

UniProt: P00511

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, L'effet Warburg