Recombinant F4/80 anticorps

N° du produit ABIN5668202

Détail du produit anti-F4/80 anticorps

Antigène: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp F4/80 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Specificité: This antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein, expressed by murine macrophages.

Attributs du produit:

OriginalSpeciesName: Rat

OriginalFormat: IgG2b

Purification: Purified antibody.

Immunogène: Thioglycollate stimulated peritoneal macrophages of mouse origin.

Clone: Cl:A3-1

Isotype: IgG2b kappa

Information d'application

Indications d'application: The anti-F4/80 antibody, clone Cl:A3-1, has been very widely used in flow cytometry, microscopy and Western blotting since its generation by Austyn and Gordon in 1980. We are the only supplier of recombinant versions of Cl:A3-1 which, whilst having the same variable domains as the original, has been shown to block hybridoma derived anti-F4/80:APC binding to in vitro generated bone marrow-derived (BMDMs) and ex vivo splenic murine macrophages. This was tested using both the recombinant version of the original isotype (rat IgG2b) as well as a murinised version (IgG2a, Ab00106-2.0). Further testing on BMDMs shows that our Fc Silent™ rat IgG2b is a valuable new reagent which shows distinct labelling of F4/80-positive cell populations, whilst the isotype control shows no binding, suggesting that only the antigen binding domains are involved in binding, promoting precision of labelling. The murinised versions will be valuable tools in the study of the role of the murine macrophage in vivo without the artefacts rat-derived antigen being co-administered may harbour. Particularly the murine IgG2a (Ab00106-2.0) should be an excellent candidate for macrophage depletion studies, with the Fc Silent™ (Ab00106-2.3) version being the ideal negative control.

Restrictions: For Research Use only

Stockage

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur F4/80

Antigène: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Autre désignation: F4/80 (EMR1 Produits)

Synonymes: anticorps EMR1, anticorps TM7LN3, anticorps DD7A5-7, anticorps EGF-TM7, anticorps F4/80, anticorps Gpf480, anticorps Ly71, anticorps adhesion G protein-coupled receptor E1, anticorps ADGRE1, anticorps LOC100424335, anticorps Adgre1

Sujet: Mouse macrophage marker, EMR1 receptor, EGF-like module, Cell surface glycoprotein F4/80, EGF-like module receptor 1, Em Gpf4, TM7, Ly-71, f480, f-480, f4 80, f 480, f-4/80, f4-80

UniProt: Q61549