COL2 anticorps
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- Antigène Voir toutes COL2 Anticorps
- COL2 (Collagen, Type II (COL2))
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Reactivité
- Boeuf (Vache)
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp COL2 est non-conjugé
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Application
- ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)
- Specificité
- Bovine collagen type II 100 % , bovine collagen XI <3.0 %, bovine collagen I and IX <0.1 % (RIA at 1:200 dilution) (cross-reacting with horse and pig)
- Réactivité croisée
- Cheval, Porc
- Réactivité croisée (Details)
- Cattle, (cross-reacting with horse and pig)
- Attributs du produit
- Type II collagen is an alpha1(II)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in cartilage and various tumours. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Bovine collagen type II 100 % , bovine collagen XI <3.0 %, bovine collagen I and IX <0.1 % (RIA at 1:200 dilution).
- Purification
- Affinity purified
- Immunogène
- Purified collagen type II from bovine cartilage
- Isotype
- IgG
- Top Product
- Discover our top product COL2 Anticorps primaire
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- Indications d'application
- After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Bovine cartilage,IHC(P), IFA, ELISA, RIA,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA 1:40, IHC(P) 1:100 - 1:500, ELISA ? 1:200 (OD ? 500)
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- 1 mg/mL
- Buffer
- phosphate buffered solution, no BSA and preservative added!
- Agent conservateur
- Without preservative
- Stock
- -20 °C
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- Antigène
- COL2 (Collagen, Type II (COL2))
- Autre désignation
- Collagen Type II (COL2 Produits)
- Synonymes
- anticorps PT, anticorps RPRGL2, anticorps THPH1, anticorps Cf-2, anticorps Cf2, anticorps FII, anticorps thrombin, anticorps prothrombin, anticorps CAP18, anticorps Cnlp, anticorps Cramp, anticorps FALL39, anticorps MCLP, anticorps coagulation factor II, thrombin, anticorps coagulation factor II, anticorps cathelicidin antimicrobial peptide, anticorps Cuticle collagen 2, anticorps collagen type II alpha 1 chain, anticorps F2, anticorps Camp, anticorps col-2, anticorps COL2A1
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