IL-3 anticorps (Biotin)
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- Antigène Voir toutes IL-3 Anticorps
- IL-3 (Interleukin 3 (IL-3))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp IL-3 est conjugé à/à la Biotin
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Application
- Western Blotting (WB), ELISA
- Purification
- Anti-hIL-3 specific antibody was purified by affinity chromatography and then biotinylated.
- Immunogène
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-3 (human IL-3).
- Top Product
- Discover our top product IL-3 Anticorps primaire
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- Indications d'application
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ELISA:
To detect hIL-3 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3.
Sandwich:
To detect hIL-3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-3 (XP-5185) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3.
Western Blot:
To detect hIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Stock
- -20 °C
- Stockage commentaire
- IL-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- Antigène
- IL-3 (Interleukin 3 (IL-3))
- Autre désignation
- IL-3 (IL-3 Produits)
- Synonymes
- anticorps BPA, anticorps Csfmu, anticorps HCGF, anticorps Il-3, anticorps MCGF, anticorps PSF, anticorps IL-3, anticorps MULTI-CSF, anticorps interleukin 3, anticorps interleukin 3 (colony-stimulating factor, multiple), anticorps Il3, anticorps IL3
- Sujet
- The pleiotropic IL-3 (Interleukin 3) is a 15 kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL-3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL-3 has also been shown to protect mast cells from undergoing apoptosis.
- ID gène
- 3562
- UniProt
- P08700
- Pathways
- Signalistation JAK/STAT, Regulation of Carbohydrate Metabolic Process, Autophagy
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