IL-4 anticorps

N° du produit ABIN5689045

Détail du produit anti-IL-4 anticorps

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-4 est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Purification: Anti-hIL-4 specific antibody was purified by affinity chromatography employing immobilized hIL-4 matrix.

Immunogène: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-4 (human Interleukin-4).

Information d'application

Indications d'application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (1.50 ng/mL), a concentration of 0.06 -0.1 μ,g/mL of this antibody is required.

ELISA:

To detect hIL-4 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-4.

Sandwich:

To detect hIL-4 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-4 (XP-5188Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-4.

Western Blot:

To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: IL-4 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur IL-4

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Autre désignation: IL-4 (IL4 Produits)

Synonymes: anticorps IL4, anticorps IL-4, anticorps BCGF-1, anticorps BCGF1, anticorps BSF-1, anticorps BSF1, anticorps Il-4, anticorps Il4e12, anticorps IL2, anticorps interleukin 4, anticorps IL4, anticorps Il4

ID gène: 3565

UniProt: P05112

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation