Profilin anticorps

N° du produit ABIN5689875

Détail du produit anti-Profilin anticorps

Antigène: Profilin (PFN)

Reactivité: Humain, Boeuf (Vache), Souris, Porc, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Profilin est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Specificité: Recognizes human, mouse, rat, pig, bovine and marsupial profilin.

Purification: Antigen affinity purified.

Immunogène: Human full-length profilin.

Information d'application

Indications d'application: ELISA: (direct or indirect: 1:2,000-1:5,000). Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05 % Tween 20 and 5 % skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: Liquid. In PBS containing 1 mg/mL BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for at least 1 year after receipt when stored at -20°C.

Détails sur Profilin

Antigène: Profilin (PFN)

Autre désignation: Profilin (PFN Produits)

Synonymes: anticorps LOC406154, anticorps GB13380, anticorps profilin, anticorps PFN, anticorps PRF

Sujet: Profilin (PFN1) is a ubiquitous small (12-15  kDa) phosphoinositide and poly-L-proline binding protein that plays a role in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels also in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes. Profilin binding to PIP2 interferes with PIP2 hydrolysis by soluble phospholipase C-gamma, an inhibition that can be overcome by tyrosine phosphorylation of PLC-gamma. Besides actin monomer sequestration and stimulation of actin nucleotide exchange, profilin can also promote cellular actin filament growth. Profilin is involved in the actin dependent intracellular motility of cytopathogenic bacteria, the regulation of cell adhesion and possibly also in linking the actin cytoskeleton and endocytosis. Profilin has been found to associate with defined complexes containing proteins such as Arp2/3 or the Rho/Rac pathways constituents ROCK-II and HEM2/NAP1. Defects in PFN1 are the cause of amyotrophic lateral sclerosis 18 (ALS18).

ID gène: 5216

NCBI Accession: NP_005013

UniProt: P07737