Produced from sera of rabbits immunized with highly pure recombinant Human VCAM-1. Human VCAM-1 specific antibody was purified by affinity chromatography employing an immobilized Human VCAM-1 matrix.
ELISA: Sandwich: To detect hVCAM-1 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Human VCAM-1 (38-281) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVCAM-1.
Western Blot:
To detect hVCAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVCAM-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions
For Research Use only
Format
Lyophilized
Stock
-20 °C
Stockage commentaire
The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.