WNT3A anticorps (Biotin)

N° du produit ABIN5690547

Détail du produit anti-WNT3A anticorps (Biotin)

Antigène: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp WNT3A est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Immunogène: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-3a. Human WNT-3a specific antibody was purified by affinity chromatography and then biotinylated.

Information d'application

Indications d'application: ELISA:
Direct:
To detect hWNT-3a by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.

Sandwich
To detect hWNT-3a by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human WNT-3a as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.

Western Blot:
To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: WNT-3a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur WNT3A

Antigène: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Autre désignation: WNT-3a (WNT3A Produits)

Synonymes: anticorps WNT3A, anticorps Wnt-3a, anticorps vt, anticorps Zwnt[a], anticorps wnt3, anticorps wnt3 l, anticorps wnt3l, anticorps wnt[a], anticorps Xwnt-3a, anticorps wnt-3a, anticorps wnt3a-A, anticorps xwnt3a, anticorps WNT-3A, anticorps WNT3, anticorps wingless-type MMTV integration site family, member 3A, anticorps Wnt family member 3A, anticorps wingless-type MMTV integration site family member 3A L homeolog, anticorps WNT3A, anticorps Wnt3a, anticorps wnt3a, anticorps wnt3a.L

Sujet: Wnt3a is a secreted ligand for members of the Frizzled family of receptors. It is one of the closely-related Wnt family and has multiple roles in development and cell fate.

ID gène: 89780

UniProt: P56704

Pathways: Signalisation WNT, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity