TOP3A anticorps

N° du produit ABIN5705925

Détail du produit anti-TOP3A anticorps

Antigène: TOP3A (Topoisomerase (DNA) III alpha (TOP3A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TOP3A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Immunogène: topoisomerase (DNA) III alpha

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Date de péremption: 12 months

Détails sur TOP3A

Antigène: TOP3A (Topoisomerase (DNA) III alpha (TOP3A))

Autre désignation: TOP3A (TOP3A Produits)

Synonymes: anticorps TOP3, anticorps DNA topoisomerase III alpha, anticorps topoisomerase (DNA) III alpha, anticorps Top3a, anticorps TOP3A

Sujet: Synonyms:TOP3 Background:Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.

ID gène: 7156

UniProt: Q13472

Pathways: Réparation de l'ADN