PARP1 anticorps (N-Term)

N° du produit ABIN5706805

N° du produit (Fournisseur): 200-401-gm8

Cet anticorps Lapin Polyclonal détecte spécifiquement PARP1 dans WB, IHC et 2D-PAGE. Il présente une réactivité envers Humain.

Aperçu rapide pour PARP1 anticorps (N-Term) (ABIN5706805)

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PARP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Two Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE)

Détail du produit anti-PARP1 anticorps

Épitope: N-Term

N° du produit (Fournisseur): 200-401-gm8

Fournisseur: Rockland

Fonction: PARP1 (N-term ZF1) Antibody

Réactivité croisée (Details): This antibody is specific for human PARP1 protein.

Attributs du produit: Synonyms: rabbit anti-PARP1 Antibody, Poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, PPOL

Purification: PARP1 (N-term ZF1) was purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads.

Stérilité: Sterile filtered

Immunogène:

Immunogen: PARP1 (N-term ZF1) purified antibody was prepared from whole rabbit serum produced by repeated immunizations with n-terminus region of human PARP1 zinc finger domain recombinant protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:100

Application Note: Anti-PARP1 (N-term ZF1) antibody has been validated by western blotting, IHC, and nanoimmunoassay (NIA). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 113 kDa in size corresponding to PARP-1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

Other: nanoimmunoassay (NIA): User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur PARP1

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Autre désignation: PARP1

Sujet: Background: PARP1 is the primary member of the poly(ADP-ribose) polymerase family, whose function is to signal DNA damage (and to recruit repair proteins) by PARylation. PARP1 is also involved in multiple cell death pathways, including apoptosis, necroptosis, autophagy, and a relatively new pathway termed parthanatos. It has been implicated in a new form of cell death termed parthanatos. PARP1 can also promote tissue survival by shifting the balance of cell death programs between autophagy and necrosis. Clinical studies have shown vulnerability to PARP inhibitors in DNA repair defective cancers. Anti-PARP1 (N-term ZF1) antibody is useful for researchers interested in cellular processes including DNA damage, transcriptional control, and stem cell identity research.

ID gène: 142

UniProt: P09874

Pathways: Apoptose, Caspase Cascade in Apoptosis, Réparation de l'ADN, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location