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Proximal-Nephrogenic Antigen / Renal Cell Carcinoma (RCC) anticorps

Reactivité: Cheval, Humain FACS, IF, IHC (f), WB Hôte: Souris Monoclonal 66-4-C2 unconjugated
N° du produit ABIN6153754
  • Antigène
    Proximal-Nephrogenic Antigen / Renal Cell Carcinoma (RCC)
    Reactivité
    Cheval, Humain
    Hôte
    • 8
    Souris
    Clonalité
    • 8
    Monoclonal
    Conjugué
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Inconjugué
    Application
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
    Fonction
    Mouse Monoclonal anti-Proximal Nephrogenic Antigen / Renal Cell Carcinoma (RCC) (66.4.C2), Purified, with BSA
    Attributs du produit
    This antibody recognizes a glycoprotein of ~200 kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Purification
    Purified
    Immunogène
    Microsomal fraction of human renal cortical tissue homogenate
    Clone
    66-4-C2
    Isotype
    IgG2b kappa
  • Indications d'application
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Commentaires

    Normal kidney or renal cell carcinoma

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    200 μg/mL
    Buffer
    PBS/0.05 % BSA/0.05 % azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Antigène
    Proximal-Nephrogenic Antigen / Renal Cell Carcinoma (RCC)
    Poids moléculaire
    55 kDa
    ID gène
    768, 63287
    UniProt
    Q16790
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