Insulin anticorps (Biotin)

N° du produit ABIN6157358

Cet anticorps anti-Insulin est un anticorps Souris Monoclonal détectant Insulin dans IF, FACS et IHC (f). Adapté pour Humain, Porc, Souris et Boeuf (Vache).

Aperçu rapide pour Insulin anticorps (Biotin) (ABIN6157358)

Antigène: Insulin (INS)

Reactivité: Humain, Porc, Souris, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Insulin est conjugé à/à la Biotin

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 2D11-H5

Détail du produit anti-Insulin anticorps (Biotin)

Fonction: Mouse Monoclonal anti-Insulin (2D11-H5), Biotin Conjugate

Attributs du produit: This antibody recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Purified pig insulin, conjugated to KLH

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.1-0.2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

MIA PaCa-2 cells or Pancreas

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur Insulin

Antigène: Insulin (INS)

Autre désignation: Insulin

Sujet: IDDM2, ILPR, IRDN, MODY10, Proinsulin

Poids moléculaire: 6 kDa

ID gène: 3630, 272259

UniProt: P01308

Pathways: Signalisation NF-kappaB, Signalisation RTK, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation