Thymidine Phosphorylase anticorps

N° du produit ABIN6165154

Détail du produit anti-Thymidine Phosphorylase anticorps

Antigène: Thymidine Phosphorylase (TYMP)

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Thymidine Phosphorylase est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Fonction: Mouse Monoclonal anti-Thymidine Phosphorylase / PD-ECGF (P-GF.44C), Purified, with BSA

Attributs du produit: This antibody recognizes a protein (amino acid 482) of 55 kDa (in vivo 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein

Clone: P-GF-44C

Isotype: IgG1

Information d'application

Indications d'application: Immunohistology (formalin) 1-2 μg/mL

• Western blotting 0.5-1 μg/mL Immunoprecipitation 0.5-1 μg/500 μg protein lysate
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Optimal dilution for a specific application should be determined by user

Commentaires:

HUVEC cells. Breast, Bladder, Lung or Kaposi tumors

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur Thymidine Phosphorylase

Antigène: Thymidine Phosphorylase (TYMP)

Autre désignation: Thymidine Phosphorylase / PD-ECGF (TYMP Produits)

Synonymes: anticorps ECGF, anticorps ECGF1, anticorps MEDPS1, anticorps MNGIE, anticorps MTDPS1, anticorps PDECGF, anticorps TP, anticorps hPD-ECGF, anticorps 2900072D10Rik, anticorps Ecgf1, anticorps PD-ECGF, anticorps Pdgfec, anticorps typh, anticorps thymidine phosphorylase, anticorps thymidine phosphorylase DeoA, anticorps AMP phosphorylase, anticorps Thymidine phosphorylase, anticorps TYMP, anticorps Tymp, anticorps deoA, anticorps Dd586_0614, anticorps Kvar_4413, anticorps NMAG_RS19330, anticorps Snov_1187, anticorps Fbal_2856, anticorps Ftrac_2071, anticorps Intca_1216, anticorps ARCVE_RS10415, anticorps Sphch_4174, anticorps Sinme_3461, anticorps METOK_RS06355, anticorps Mesop_1831, anticorps typh

Sujet: ECGF, ECGF1, Gliostatin, hPD-ECGF, MEDPS1, MNGIE, MTDPS1, PD-ECGF, PDECGF, Platelet-derived endothelial cell growth factor, TdRPase, Thymidine phosphorylase, TP, Tymp

Poids moléculaire: 55 kDa

ID gène: 1890, 180903

UniProt: P19971

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2