PLAP anticorps

N° du produit ABIN6251805

Détail du produit anti-PLAP anticorps

Antigène: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PLAP est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogène: Recombinant full-length human ALPP protein was used as the immunogen for the placental Alkaline Phosphatase antibody.

Clone: ALPP-870

Isotype: IgG2b kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the placental Alkaline Phosphatase antibody should be determined by the researcher.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur PLAP

Antigène: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Autre désignation: Placental Alkaline Phosphatase (ALPP Produits)

Synonymes: anticorps ECK0378, anticorps JW0374, anticorps psiA, anticorps ALP, anticorps PALP, anticorps PLAP, anticorps PLAP-1, anticorps ILC, anticorps CTACK, anticorps ESkine, anticorps Akp2, anticorps alp, anticorps zgc:56672, anticorps DDBDRAFT_0205491, anticorps DDBDRAFT_0231570, anticorps DDB_0205491, anticorps DDB_0231570, anticorps ALPI, anticorps Actn2lp, anticorps Alp, anticorps bacterial alkaline phosphatase, anticorps alkaline phosphatase, placental, anticorps alkaline phosphatase, placental type, anticorps C-C motif chemokine ligand 27, anticorps alkaline phosphatase, liver/bone/kidney, anticorps alkaline phosphatase, anticorps alkaline phosphatase, intestinal, anticorps PDZ and LIM domain 3, anticorps phoA, anticorps ALPP, anticorps LOC100436725, anticorps CCL27, anticorps alpl, anticorps alp, anticorps ALPI, anticorps Pdlim3, anticorps Alpp

Sujet: Reacts with a 70  kDa membrane-bound isozyme (Regan and Nagao type) of placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

ID gène: 250

UniProt: P05187