Chromogranin A anticorps

N° du produit ABIN6251930

Détail du produit anti-Chromogranin A anticorps

Antigène: Chromogranin A (CHGA)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Chromogranin A est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogène: Recombinant human protein was used as the immunogen for the Chromogranin A antibody.

Clone: BGRAN9

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Western blot: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the Chromogranin A antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur Chromogranin A

Antigène: Chromogranin A (CHGA)

Autre désignation: Chromogranin A (CHGA Produits)

Synonymes: anticorps CGA, anticorps CG-ALPHA, anticorps FSHA, anticorps GPHA1, anticorps GPHa, anticorps HCG, anticorps LHA, anticorps TSHA, anticorps ChrA, anticorps fj05f09, anticorps si:dkey-177p2.2, anticorps wu:fj05f09, anticorps zgc:101749, anticorps zgc:56075, anticorps CHGA, anticorps cgA, anticorps chga, anticorps chromogranin A, anticorps glycoprotein hormones, alpha polypeptide, anticorps uncharacterized CHGA, anticorps chromogranin A S homeolog, anticorps CHGA, anticorps CGA, anticorps Chga, anticorps chga, anticorps chga.S

Sujet: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

ID gène: 1113

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling