p40 (p63 Delta) (AA 5-17) anticorps

N° du produit ABIN6252140

Détail du produit

Antigène: p40 (p63 Delta)

Épitope: AA 5-17

Reactivité: Humain, Boeuf (Vache), Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Purification: Protein A affinity chromatography

Immunogène: Amino acids ENNAQTQFSEPQY (aa 5-17) of human p40 / p63 delta were used as the immunogen for this antibody.

Isotype: IgG

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/10e6 cells

IF: 1-2 μg/mL

WB: 0.5-1 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p40 antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues requires boiling sections in 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détail du antigène

Antigène: p40 (p63 Delta)

Autre désignation: p40

Synonymes: anticorps AIS, anticorps B(p51A), anticorps B(p51B), anticorps EEC3, anticorps KET, anticorps LMS, anticorps NBP, anticorps OFC8, anticorps RHS, anticorps SHFM4, anticorps TP53CP, anticorps TP53L, anticorps TP73L, anticorps p40, anticorps p51, anticorps p53CP, anticorps p63, anticorps p73H, anticorps p73L, anticorps tumor protein p63, anticorps TP63

Sujet: P40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p40 antibody's ability to distinguish between the tumor types appears to be superior when compared to p63. The ability to utilize a p40 antibody probe as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.

ID gène: 8626

UniProt: Q9H3D4