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Calponin anticorps

Reactivité: Humain, Rat IHC (p), IF Hôte: Souris Monoclonal CLPN5-1 unconjugated
N° du produit ABIN6252460
  • Antigène Tous les produits Calponin
    Calponin
    Reactivité
    • 39
    • 33
    • 24
    • 8
    • 8
    • 7
    • 5
    • 3
    • 1
    • 1
    • 1
    Humain, Rat
    Hôte
    • 24
    • 16
    Souris
    Clonalité
    • 28
    • 12
    Monoclonal
    Conjugué
    • 25
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Calponin est non-conjugé
    Application
    • 18
    • 18
    • 17
    • 12
    • 12
    • 11
    • 9
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Purification
    Protein G affinity chromatography
    Immunogène
    Recombinant human protein was used as the immunogen for the Calponin antibody.
    Clone
    CLPN5-1
    Isotype
    IgG1 kappa
  • Indications d'application
    Immunofluorescence: 1-2 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    Optimal dilution of the Calponin antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 mg/mL
    Buffer
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Antigène
    Calponin
    Abstract
    Calponin Produits
    Synonymes
    anticorps CalPoNin, anticorps cpn-1, anticorps cpn-2
    Sujet
    Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
    ID gène
    1264
    UniProt
    P51911
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