CA9 anticorps
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- Antigène Voir toutes CA9 Anticorps
- CA9 (Carbonic Anhydrase IX (CA9))
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Reactivité
- Humain, Cheval
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CA9 est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Purification
- Protein G affinity chromatography
- Immunogène
- The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for this Carbonic Anhydrase IX / CAIX antibody.
- Clone
- 66-4-C2
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product CA9 Anticorps primaire
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- Indications d'application
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Flow Cytometry: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CAIX antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Antigène
- CA9 (Carbonic Anhydrase IX (CA9))
- Autre désignation
- CAIX (CA9 Produits)
- Synonymes
- anticorps CA9, anticorps CAIX, anticorps Ca9, anticorps MN/CA9, anticorps MN, anticorps carbonic anhydrase 9, anticorps CA9, anticorps CAH9, anticorps Car9
- Sujet
- This antibody recognizes a glycoprotein of ~200 kDa, identified as renal cell carcinoma protein, carbonic anhydrase IX / CAIX, or gp200. Its epitope resides in the carbohydrate domain of the protein. The CAIX antibody shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, CAIX is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, it is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This CAIX antibody may be useful in the investigations of carcinomas of proximal nephrogenic differentiation, especially those showing tubular differentiation.
- ID gène
- 768
- UniProt
- Q16790
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