Prostate Specific Antigen anticorps
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- Antigène Voir toutes Prostate Specific Antigen (PSA) Anticorps
- Prostate Specific Antigen (PSA)
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Prostate Specific Antigen est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Purification
- Protein G affinity chromatography
- Immunogène
- Prostate specific antigen from human sperm plasma was used as the immunogen for this PSA antibody.
- Clone
- SPM352
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product PSA Anticorps primaire
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- Indications d'application
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the PSA antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Antigène
- Prostate Specific Antigen (PSA)
- Autre désignation
- PSA (PSA Produits)
- Synonymes
- anticorps APS, anticorps KLK2A1, anticorps PSA, anticorps hK3, anticorps KLK3, anticorps Klk1c10l2, anticorps RSGK-50, anticorps RSKG-50, anticorps rGK-3, anticorps Kal-3, anticorps Kal3, anticorps Klk3, anticorps AAP-S, anticorps MP100, anticorps Psa, anticorps R74825, anticorps goku, anticorps kallikrein related peptidase 3, anticorps kallikrein 3, anticorps kallikrein 1-related peptidase C3, anticorps kallikrein B, plasma 1, anticorps aminopeptidase puromycin sensitive, anticorps KLK3, anticorps Klk1c3, anticorps Klkb1, anticorps Npepps
- Sujet
- Recognizes a single protein of 33-34 kDa, identified as the prostate specific antigen (PSA). This mAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.
- ID gène
- 354
- UniProt
- P07288
- Pathways
- Système du Complément
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