TP53BP1 anticorps (pThr543)

N° du produit ABIN6256578

L’anticorps Lapin Polyclonal anti-TP53BP1 a été validé pour WB, ELISA, IF et ICC. Il convient pour détecter TP53BP1 dans des échantillons de Humain et Singe.

Aperçu rapide pour TP53BP1 anticorps (pThr543) (ABIN6256578)

Antigène: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Reactivité: Humain, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TP53BP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-TP53BP1 anticorps

Épitope: pThr543

Specificité: Phospho-53BP1 (Thr543) Antibody detects endogenous levels of 53BP1 only when phosphorylated at Thr543

Réactivité croisée: Humain, Singe

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogène: A synthesized peptide derived from human Phospho-53BP1 (Thr543)

Isotype: IgG

Information d'application

Indications d'application: WB 1:1000-3000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C.Stable for 12 months from date of receipt

Date de péremption: 12 months

Détails sur TP53BP1

Antigène: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Autre désignation: 53BP1

Sujet:

Description: Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:28241136). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:28241136, PubMed:17190600). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates to the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).

Gene: TP53BP1

Poids moléculaire: 220kDa

ID gène: 7158

UniProt: Q12888

Pathways: Réparation de l'ADN