RENT1/UPF1 anticorps

N° du produit ABIN6266288

Détail du produit anti-RENT1/UPF1 anticorps

Antigène: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RENT1/UPF1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: UPF1 Antibody detects endogenous levels of total UPF1

Réactivité croisée: Humain, Souris, Rat (Rattus)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthetic peptide of human UPF1

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C.Stable for 12 months from date of receipt

Date de péremption: 12 months

Détails sur RENT1/UPF1

Antigène: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Autre désignation: UPF1 (UPF1 Produits)

Synonymes: anticorps HUPF1, anticorps NORF1, anticorps RENT1, anticorps pNORF1, anticorps smg-2, anticorps B430202H16Rik, anticorps PNORF-1, anticorps Rent1, anticorps Upflp, anticorps rent1, anticorps wu:fi40f07, anticorps wu:fj48a01, anticorps zgc:55472, anticorps Tb05.3C6.50, anticorps AO090012000584, anticorps hupf1, anticorps norf1, anticorps pnorf1, anticorps upf1, anticorps UPF1, RNA helicase and ATPase, anticorps UPF1 regulator of nonsense transcripts homolog (yeast), anticorps upf1 regulator of nonsense transcripts homolog (yeast), anticorps regulator of nonsense transcripts 1, anticorps Regulator of nonsense transcripts 1, anticorps UPF1 regulator of nonsense transcripts homolog S homeolog, anticorps UPF1, anticorps Upf1, anticorps upf1, anticorps Tc00.1047053511317.30, anticorps Tb927.5.2140, anticorps TVAG_453890, anticorps TVAG_237840, anticorps AOR_1_1018194, anticorps HPB8_739, anticorps smg-2, anticorps upf1.S

Sujet:

Description: RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation, its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S, the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability.

Gene: UPF1

Poids moléculaire: 123kDa

ID gène: 5976

UniProt: Q92900

Pathways: SARS-CoV-2 Protein Interactome