p53 anticorps (N-Term)

N° du produit ABIN6295698

Cet anticorps anti-p53 Monoclonal Souris (ABIN6295698) détecte spécifiquement p53 dans WB, IHC, IF et FACS. L’anticorps est réactif avec des échantillons de Humain, Singe et Boeuf (Vache).

Aperçu rapide pour p53 anticorps (N-Term) (ABIN6295698)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain, Singe, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-p53 anticorps

Épitope: AA 20-25, N-Term

Fonction: Mouse anti-Human/Monkey/Cow p53 Antibody [Sodium Azide Free]

Specificité: Nuclear

Purification: Recombinant human wild type p53 expressed in E. coli was used as the immunogen for this antibody. Its epitope maps within the N-terminus portion (aa 20-25) of the p53 oncoprotein.

Immunogène: Recombinant human wild type p53 expressed in E. coli was used as the immunogen for this antibody. Its epitope maps within the N-terminus portion (aa 20-25) of the p53 oncoprotein.

Information d'application

Indications d'application: FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.5-1.0 μg/mL
IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: Cellular tumor antigen p53

Sujet:

Target Description: This antibody is specific for a 53 kDa protein, which is identified as p53 suppressor gene product. The large number of prolines in its amino acid sequence causes p53 to migrate slowly in SDS-PAGE, resulting in the amino acid content-estimated 43 kDa protein appearing larger than expected. DO-7 reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intra-tubular germ cell neoplasia.

Gene Symbol: TP53///P53

ID gène: 7157

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg