SLC7A11 anticorps

N° du produit ABIN6655336

Détail du produit anti-SLC7A11 anticorps

Antigène: SLC7A11 (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SLC7A11 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Purification: This affinity purified antibody is directed against human xCT. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human and orangutan, and 88% identity to mouse.

Immunogène:

Immunogen: Anti-xCT antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human xCT conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200

Application Note: Anti-xCT Antibody has been tested in Western Blot, ELISA, Immunofluorescence and Flow cytometry. Expect a band at 55.4 kDa in western blot using appropriate lysates. Positive control used NIH-3T3, Hela, HCT-116, A549, or PC3 whole cell lysates at 0.1-1.0 μg/mL for WB and PC3 with MeOH at 10 μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 5.0 μg/mL

Flow Cytometry Dilution: 1:200

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 10 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Détails sur SLC7A11

Antigène: SLC7A11 (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11))

Autre désignation: xCT (SLC7A11 Produits)

Synonymes: anticorps SLC7A11, anticorps DKFZp468E122, anticorps CCBR1, anticorps xCT, anticorps 9930009M05Rik, anticorps AI451155, anticorps sut, anticorps solute carrier family 7 member 11, anticorps solute carrier family 7, (cationic amino acid transporter, y+ system) member 11, anticorps solute carrier family 7 (cationic amino acid transporter, y+ system), member 11, anticorps SLC7A11, anticorps Slc7a11

Sujet:

Synonyms: rabbit anti-xCT antibody, SLC7A11, Cystine/glutamate transporter, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, Solute carrier family 7 member 11

Background: xCT belongs to the amino acid-polyamine-organocation (APC) superfamily and L-type amino acid transporter (LAT) family. xCT gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. Increased expression of this gene results in neuronal cell death. xCT may be associated with kaposi sarcoma, dyscalculia, cystinuria, spondylolsis, and anemia of prematurity. Anti-xCT Antibody is useful for researchers interested in Cancer Research, Glucose/Energy Metabolism Research, and Cell Surface Protein Interaction Research.

Gene Name: SLC7A11

ID gène: 23657

NCBI Accession: NP_055146

UniProt: Q9UPY5