CHEK1 anticorps (AA 192-241)

N° du produit ABIN6736365

Cet anticorps anti-CHEK1 est un anticorps Lapin Polyclonal détectant CHEK1 dans WB, IHC et IHC (p). Adapté pour Humain, Souris, Rat, Chien, Lapin, Cheval, Singe, Poisson zèbre (Danio rerio) et Roussette (Chauve-souris).

Aperçu rapide pour CHEK1 anticorps (AA 192-241) (ABIN6736365)

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivité: Humain, Souris, Rat, Chien, Lapin, Cheval, Singe, Poisson zèbre (Danio rerio), Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHEK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-CHEK1 anticorps

Épitope: AA 192-241

Marque: IHC-plus™

Specificité: Human CHEK1 / CHK1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Dog, Horse (100%) Bovine, Pig, Guinea pig, Chicken (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa192-241 of human CHEK1 (O14757, NP_001265). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Rabbit, Horse, Zebra finch (100%), Hamster, Bovine, Pig, Opossum, Guinea pig, Chicken, Platypus (92%), Stickleback (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur CHEK1

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Autre désignation: CHEK1 / CHK1

Sujet: Name/Gene ID: CHEK1
Subfamily: NIM1
Family: Protein Kinase

Synonyms: CHEK1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase 1, CHK1, Chk1-S, Protein kinase chk1, Checkpoint kinase-1

ID gène: 1111

NCBI Accession: NP_001265

UniProt: O14757

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire, Réparation de l'ADN