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BAD anticorps (AA 101-204)

BAD Reactivité: Humain, Souris, Rat WB, ELISA, IHC (p), IF (p), IF (cc), FACS, ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN674709
  • Antigène Voir toutes BAD Anticorps
    BAD (BCL2-Associated Agonist of Cell Death (BAD))
    Épitope
    • 34
    • 34
    • 27
    • 27
    • 26
    • 24
    • 24
    • 23
    • 15
    • 11
    • 11
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    AA 101-204
    Reactivité
    • 384
    • 223
    • 146
    • 17
    • 9
    • 6
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 459
    • 16
    • 1
    Lapin
    Clonalité
    • 441
    • 35
    Polyclonal
    Conjugué
    • 181
    • 43
    • 38
    • 38
    • 36
    • 33
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 6
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Cet anticorp BAD est non-conjugé
    Application
    • 257
    • 257
    • 163
    • 105
    • 80
    • 79
    • 56
    • 54
    • 21
    • 20
    • 12
    • 10
    • 10
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
     Réactivité croisée
    Humain, Souris, Rat
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from human BAD
    Isotype
    IgG
  • Indications d'application
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Restrictions
    For Research Use only
  • Validation #029802 (Western Blotting)
    'Independent Validation' signe
    by
    Alamo Laboratories Inc
    No.
    #029802
    Date
    26.08.2014
    Antigène
    Numéro du lot
    090915
    Application validée
    Western Blotting
    Contrôle positif
    MCF7 cells
    Conclusion
    A strong band was observed in the positive control sample at the correct molecular weight. No bands were observed in the negative control sample.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    • Antigen: BCL2-Associated Agonist of Cell Death (BAD)
    • Catalog number: ABIN674709
    • Lot number: 090915
    • Antibody Dilution: 1:200
    Anticorps secondaire
    • Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
    • Lot number: L170-6515
    • Antibody Dilution: 1:10,000
    Full Protocol
    • 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
    • 2. 15 μl of heated extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
    • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
    • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
    • 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
    • 6. The membrane was rinsed with TBST once.
    • 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 16 hours at 4°C.
    • 8. The membrane was rinsed in TBST thrice for 5 minutes each.
    • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
    • 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
    • 11. The membrane was rinsed in TBS twice for 30 seconds each.
    • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 600 seconds.
    • 13. The membrane was rinsed three times TBST.
    • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
    • 15. The membrane was washed in TBST 2 times for 10 minutes each.
    • 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
    Notes
    - No experimental challenges noted.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Antigène
    BAD (BCL2-Associated Agonist of Cell Death (BAD))
    Autre désignation
    Bad (BAD Produits)
    Synonymes
    anticorps bad, anticorps fa01b12, anticorps wu:fa01b12, anticorps wu:fa96d04, anticorps BAD, anticorps BBC2, anticorps BCL2L8, anticorps AI325008, anticorps Bbc2, anticorps BCL2-associated agonist of cell death b, anticorps BCL2 associated agonist of cell death, anticorps BCL2-associated agonist of cell death, anticorps badb, anticorps BAD, anticorps Bad
    Sujet

    Synonyms: BBC2, BCL2L8, Bcl2-associated agonist of cell death, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, BBC6

    Background: Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

    ID gène
    572
    UniProt
    Q92934
    Pathways
    Signalisation MAPK, Signalisation PI3K-Akt, Signalisation RTK, Apoptose, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events
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