CDKN1C anticorps

N° du produit ABIN6936916

Détail du produit anti-CDKN1C anticorps

Antigène: CDKN1C (Cyclin-Dependent Kinase Inhibitor 1C (p57, Kip2) (CDKN1C))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDKN1C est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Recognizes a protein of 57 kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.

Réactivité croisée (Details): Human and Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human p57Kip2 protein

Clone: 57P06

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: LS174T, Raji, HT29, SK-BR3 cells. Human colon or prostate carcinomas.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CDKN1C

Antigène: CDKN1C (Cyclin-Dependent Kinase Inhibitor 1C (p57, Kip2) (CDKN1C))

Autre désignation: CDKN1C (CDKN1C Produits)

Synonymes: anticorps BWCR, anticorps BWS, anticorps KIP2, anticorps WBS, anticorps p57, anticorps p57Kip2, anticorps cb961, anticorps p27Xic1-a, anticorps zgc:86618, anticorps AL024410, anticorps CDKI, anticorps Kip2, anticorps p57(kip2), anticorps p57KIP2, anticorps cyclin dependent kinase inhibitor 1C, anticorps cyclin-dependent kinase inhibitor 1Ca, anticorps cyclin-dependent kinase inhibitor 1C (P57), anticorps cyclin-dependent kinase inhibitor 1C, anticorps CDKN1C, anticorps cdkn1ca, anticorps Cdkn1c

Sujet: Beckwith Wiedemann syndrome (WBS), BWCR, Cyclin dependent kinase inhibitor 1C (CDKN1C), KIP2, p57,p57Kip2 (Mitotic Inhibitor/Suppressor Protein)
Cellular localisation: Nuclear

Poids moléculaire: 57kDa

ID gène: 1028, 106070

UniProt: P49918

Pathways: Cycle Cellulaire, Dopaminergic Neurogenesis