GAD65 anticorps (AA 6-99)

N° du produit ABIN6937293

Détail du produit anti-GAD65 anticorps

Antigène: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Épitope: AA 6-99

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GAD65 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: This MAb recognizes a protein of 65 kDa, which is identified as glutamic acid decarboxylase 2 (GDA2). It is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. There are two forms of glutamic acid decarboxylases (GAD s) that are found in the brain: GAD2 (also known as GAD65) and GAD1 (also known as GAD67). GAD1 and GAD2 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD2 localizes to synaptic vesicle membranes in nerve terminals, while GAD1 is distributed throughout the cell. A pathogenic role for GAD2 is identified in the human pancreas since it has been identified as an autoantibody and an auto-reactive T cell target in insulin-dependent diabetes.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human GAD2 (GAD65) protein fragment (around aa 6-99) (exact sequence is proprietary)

Clone: GAD2-1960

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Pancreas or Brain (IHC).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur GAD65

Antigène: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Autre désignation: GAD2 (GAD2 Produits)

Synonymes: anticorps GAD65, anticorps gad65, anticorps F12P19.12, anticorps F12P19_12, anticorps GLUTAMATE DECARBOXYLASE 2, anticorps glutamate decarboxylase 2, anticorps zgc:112198, anticorps 6330404F12Rik, anticorps GAD(65), anticorps Gad-2, anticorps glutamate decarboxylase 2, anticorps glutamate decarboxylase 1, anticorps glutamic acid decarboxylase 2, anticorps GAD2, anticorps PTRG_06148, anticorps LOC9324534, anticorps Gad2, anticorps GAD1, anticorps gad2

Sujet: DCE2, GAD2, Glutamic Acid Decarboxylase 2 (Pancreas), Glutamic Acid Decarboxylase 2 (Pancreatic Islets and Brain 65 kDa), Glutamic Acid Decarboxylase 65 (GAD65),GAD2 / GAD65 (GABAergic Neuronal Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 65kDa

ID gène: 2572, 231829

UniProt: Q05329