MCM6 anticorps (AA 228-368)

N° du produit ABIN6937817

Cet anticorps Souris Monoclonal détecte spécifiquement MCM6 dans IHC et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour MCM6 anticorps (AA 228-368) (ABIN6937817)

Antigène: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MCM6 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: MCM6-2999

Détail du produit anti-MCM6 anticorps

Épitope: AA 228-368

Specificité: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM6 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1/S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the post-replicative state of the cell. MCM2/MCM6 complexes function as substrates for Cdc2/cyclin B in vitro.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (around aa 228-368) of human MCM6 protein (exact sequence is proprietary)

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Human skin or squamous cell carcinoma (IHC).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur MCM6

Antigène: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Autre désignation: MCM6

Sujet: DNA replication licensing factor MCM6, MCM6 minichromosome maintenance deficient 6, MIS5 homolog, p105MCM,MCM6 (Proliferation Marker)
Cellular localisation: Nuclear

Poids moléculaire: 105kDa

ID gène: 4175, 444118

UniProt: Q14566

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA