Insulin anticorps

N° du produit ABIN6939789

L’anticorps anti-Insulin Monoclonal Souris est utilisé pour la détection de Insulin dans des échantillons de Humain, Porc et Boeuf (Vache). Il a été validé pour IHC, IF et FACS.

Aperçu rapide pour Insulin anticorps (ABIN6939789)

Antigène: Insulin (INS)

Reactivité: Humain, Porc, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Insulin est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 2D11-H5-same as INS05

Détail du produit anti-Insulin anticorps

Fonction: Insulin / IRDN (beta-Cell & Insulinoma Marker) Antibody

Specificité: Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Purified pig insulin, conjugated to KLH

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: MIA PaCa-2 cells. Pancreas.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 minutes at RT)(No special pretreatment is required for staining of formalin/paraffin tissues.)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Insulin

Antigène: Insulin (INS)

Autre désignation: INS

Sujet:

IDDM2, ILPR, IRDN, MODY10, Proinsulin

MIA PaCa-2 cells. Pancreas.

Poids moléculaire: 6kDa

ID gène: 3630, 272259

UniProt: P01308

Pathways: Signalisation NF-kappaB, Signalisation RTK, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation