Recombinant p53 anticorps

N° du produit ABIN6940792

Cet anticorps Lapin Monoclonal détecte spécifiquement p53 dans WB, IHC et StM. Il présente une réactivité envers Humain, Singe, Chien, Hamster et Poulet.

Aperçu rapide pour Recombinant p53 anticorps (ABIN6940792)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Singe, Chien, Hamster, Poulet

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: TP53-2092R

Détail du produit anti-p53 anticorps

Specificité: This MAb reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 Molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70 % but incidence varies from zero in carcinoid lung tumors to 97 % in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.

Aucune reactivité croisée: Souris, Rat (Rattus)

Immunogène: Recombinant full-length human TP53 protein

Isotype: IgG

Information d'application

Indications d'application:

Positive Control: MDA-MB-231 cells. Breast or Colon carcinoma.

Known Application: Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: TP53

Poids moléculaire: 53kDa

ID gène: 7157

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg