Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Purification
TRAF6 Antibody is Ion exchange chromatography purified.
Immunogène
TRAF6 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human TRAF6. The immunogen is located within the last 50 amino acids of TRAF6.
TRAF6
Reactivité: Humain
WB, IF
Hôte: Souris
Polyclonal
unconjugated
Indications d'application
TRAF6 antibody can be used for detection of TRAF6 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
TRAF6 Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
TRAF6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
TRAF6 Antibody: Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-κ,B. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands. It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK.