TOP1MT anticorps

N° du produit ABIN7120014

Détail du produit anti-TOP1MT anticorps

Antigène: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TOP1MT est non-conjugé

Application: Western Blotting (WB), ELISA

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: topoisomerase(DNA) I, mitochondrial

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:2000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Date de péremption: 12 months

Détails sur TOP1MT

Antigène: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Autre désignation: TOP1MT (TOP1MT Produits)

Synonymes: anticorps 2900052H09Rik, anticorps TOP1, anticorps topoisomerase (DNA) I, mitochondrial, anticorps DNA topoisomerase 1, mitochondrial, anticorps DNA topoisomerase I mitochondrial, anticorps TOP1MT, anticorps Top1mt

Sujet: Synonyms: Background:Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone(By similarity).

Poids moléculaire: 70 kDa

ID gène: 116447

UniProt: Q969P6