FZD7 anticorps (Frizzled Family Receptor 7) (AA 550-574)

Details for Product anti-FZD7 Antibody No. ABIN710051
Antigène
  • FzE3
  • Fz-7
  • Xfz7
  • frizzled-7
  • frizzled7
  • frz-7
  • frz7
  • fz7
  • fzd7-a
  • fzd7-b
  • fze3
  • Fz7
  • cFz-7
  • fb38g02
  • fc44b09
  • fz13
  • fz7a
  • fz7b
  • wu:fb38g02
  • wu:fc44b09
  • zg13
  • frizzled class receptor 7
  • frizzled class receptor 7 L homeolog
  • frizzled class receptor 7b
  • FZD7
  • fzd7.L
  • fzd7
  • Fzd7
  • fzd7b
Épitope
AA 550-574
24
18
14
11
10
8
8
7
6
2
2
2
2
2
2
2
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
101
82
24
5
3
3
2
2
2
1
1
1
1
1
Hôte
Lapin
106
12
11
7
Clonalité
Polyclonal
Conjugué
Cet anticorp FZD7 est non-conjugé
9
5
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
101
62
18
16
12
12
4
2
2
1
1
1
Options
'Independent Validation' signe
Numéro du lot 120426
Application validée Western Blotting
Contrôle positif MDA-MB-231 cells
Contrôle négative C6/36 cells (non-reactive species)
Conclusion A strong band was observed in the positive control sample at the expected molecular weight of ~64 kDa. The band is also faintly observed in the negative control sample, which may indicate species cross-reactivity.
Anticorps primaire
  • Antigen: Frizzled Family Receptor 7 (FZD7)
  • Catalog number: ABIN710051
  • Lot number: 120426
  • Antibody Dilution: 1:100
Anticorps secondaire
  • Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
  • Lot number: L170-6515
  • Antibody Dilution: 1:10,000
Contrôles
  • Positive control: MDA-MB-231 cells
  • Negative control: C6/36 cells
Protocole
  • 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
  • 2. 15 μl of heated culture-media were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 24 hours at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 minutes each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
  • 11. The membrane was rinsed in TBS twice for 30 seconds each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 45 minutes.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
  • 15. The membrane was washed in TBST 2 times for 10 minutes each.
  • 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
Notes - No experimental challenges noted.
Validation Images
Western Blotting validation image for anti-Frizzled Family Receptor 7 (FZD7) (AA 550-574) antibody (ABIN710051) Figure 1: Western Blot for FZD7. Arrowhead indicates the expected molecular weight of...
Immunogène KLH conjugated synthetic peptide derived from human Frizzled 7
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others FZD7 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation Frizzled 7
Sujet

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Subcellular location: Cytoplasm

Synonyms: FzE3, Frizzled-7, Fz-7, hFz7, FZD7

ID gène 8324
UniProt O75084
Pathways Signalisation WNT, Stem Cell Maintenance
Indications d'application WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store at -20°C
Date de péremption 12 months
Images (Fournisseur)
Image no. 1 for anti-Frizzled Family Receptor 7 (FZD7) (AA 550-574) antibody (ABIN710051) Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Frizzled ...
Image no. 2 for anti-Frizzled Family Receptor 7 (FZD7) (AA 550-574) antibody (ABIN710051) Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Frizzled...
Image no. 3 for anti-Frizzled Family Receptor 7 (FZD7) (AA 550-574) antibody (ABIN710051) Image provided by the Independent Validation Program (badge number 29805). Lane 1: MD...
Produit citée dans: Deng, Zhang, Zhang, Wen, Miao, Guo: "MicroRNA-142-3p inhibits cell proliferation and invasion of cervical cancer cells by targeting FZD7." dans: Tumour biology, 2015 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

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