POLD1 anticorps (Catalytic Subunit) (HRP)

N° du produit ABIN7150230

Détail du produit anti-POLD1 anticorps (HRP)

Antigène: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Épitope: AA 1-123, Catalytic Subunit

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLD1 est conjugé à/à la HRP

Application: ELISA

Réactivité croisée: Humain

Purification: >95%, Protein G purified

Immunogène: Recombinant Human DNA polymerase delta catalytic subunit protein (1-123AA)

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur POLD1

Antigène: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Autre désignation: POLD1 (POLD1 Produits)

Synonymes: anticorps CDC2, anticorps CRCS10, anticorps POLD, anticorps 5949, anticorps CAA61369, anticorps CG5949, anticorps DNA Pol Delta, anticorps Dm Poldelta, anticorps Dmel\\CG5949, anticorps Poldelta, anticorps dnapol delta, anticorps l(3)72Ac, anticorps cdc2, anticorps pold, anticorps 125kDa, anticorps Pol delta, anticorps fla, anticorps im:7139126, anticorps zgc:152688, anticorps DNA polymerase delta 1, catalytic subunit, anticorps DNA-polymerase-delta, anticorps polymerase (DNA directed), delta 1, catalytic subunit, anticorps DNA polymerase delta catalytic subunit, anticorps DNA polymerase delta, anticorps POLD1, anticorps DNApol-delta, anticorps pold1, anticorps LOC100456571, anticorps Pold1

Sujet:

Background: As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3\'- to 5\'-exonuclease activities (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200, PubMed:24022480). Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5\'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed:19074196).

Aliases: Polymerase (DNA directed) delta 1 catalytic subunit antibody, CDC2 antibody, CDC2 homolog antibody, CRCS10 antibody, DNA directed DNA polymerase delta 1 antibody, DNA directed polymerase delta 1 antibody, DNA pol delta 1 antibody, DNA polymerase delta catalytic subunit antibody, DNA polymerase subunit delta p125 antibody, DPOD1_HUMAN antibody, MDPL antibody, POLD antibody, POLD 1 antibody, POLD1 antibody, Polymerase (DNA directed) delta 1 catalytic subunit 125 kDa antibody, Polymerase (DNA) delta 1 catalytic subunit antibody, Polymerase DNA directed delta 1 catalytic subunit 125kD antibody, polymerase, DNA, delta antibody

UniProt: P28340

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Chromatin Binding, Synthesis of DNA