POLD3 anticorps (AA 203-275) (HRP)

N° du produit ABIN7150238

Détail du produit anti-POLD3 anticorps (HRP)

Antigène: POLD3 (Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3))

Épitope: AA 203-275

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLD3 est conjugé à/à la HRP

Application: ELISA

Réactivité croisée: Humain

Purification: >95%, Protein G purified

Immunogène: Recombinant Human DNA polymerase delta subunit 3 protein (203-275AA)

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur POLD3

Antigène: POLD3 (Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3))

Autre désignation: POLD3 (POLD3 Produits)

Synonymes: anticorps zgc:136471, anticorps P66, anticorps P68, anticorps 2410142G14Rik, anticorps C85233, anticorps polymerase (DNA-directed), delta 3, accessory subunit L homeolog, anticorps polymerase (DNA-directed), delta 3, accessory subunit, anticorps DNA polymerase delta 3, accessory subunit, anticorps pold3.L, anticorps pold3, anticorps POLD3, anticorps Pold3

Sujet:

Background: As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA (PubMed:10219083, PubMed:10852724, PubMed:11595739, PubMed:16510448, PubMed:24035200). Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5\'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites (PubMed:20227374). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion (PubMed:19074196, PubMed:25628356, PubMed:27185888). Also involved in TLS, as a component of the POLZ complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (PubMed:24449906).

Aliases: DNA polymerase delta subunit 3 antibody, DNA polymerase delta subunit p66 antibody, DNA polymerase delta, subunit 3 antibody, DNA polymerase subunit delta p66 antibody, DPOD3_HUMAN antibody, KIAA0039 antibody, P66 antibody, P68 antibody, Pol delta C subunit (p66) antibody, Pold3 antibody, Polymerase (DNA directed), delta 3 antibody, Polymerase (DNA-directed), delta 3, accessory subunit antibody, PPP1R128 antibody, Protein phosphatase 1, regulatory subunit 128 antibody

UniProt: Q15054

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA