RICTOR anticorps (AA 1-50)

N° du produit ABIN7454507

Cet anticorps anti-RICTOR est un anticorps Lapin Polyclonal détectant RICTOR dans IP et IHC (fp). Adapté pour Humain.

Aperçu rapide pour RICTOR anticorps (AA 1-50) (ABIN7454507)

Antigène: RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RICTOR est non-conjugé

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Détail du produit anti-RICTOR anticorps

Épitope: AA 1-50

Fonction: Rabbit anti-Rictor Antibody, Affinity Purified

Purification: Affinity Purified

Immunogène: Between AA 1 and 50

Isotype: IgG

Information d'application

Indications d'application:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended. A304-329A has not performed satisfactorily when used for WB of Rictor in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Rictor.

Restrictions: For Research Use only

Stockage

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Date de péremption: 12 months

Détails sur RICTOR

Antigène: RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))

Autre désignation: Rictor

Sujet: Background: Rictor is a subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development [taken from the Universal Protein Resource (UniProt) Q6R327].

ID gène: 253260

NCBI Accession: NP_689969

UniProt: Q6R327

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events