PARP1 anticorps

N° du produit ABIN7464127

L’anticorps Souris Monoclonal anti-PARP1 a été validé pour WB, IP et IHC (p). Il convient pour détecter PARP1 dans des échantillons de Humain.

Aperçu rapide pour PARP1 anticorps (ABIN7464127)

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PARP1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: KO Validated

Clone: GT6212

Détail du produit anti-PARP1 anticorps

Specificité: IP/MS validation was supported by references (PMID:30377422)

Réactivité croisée: Humain, Souris, Rat

Purification: Affinity purified by Protein G.

Immunogène: Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.

Isotype: IgG2b

Information d'application

Indications d'application: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: 293T , A431 , HeLa , HepG2 , A375 , HCT116 (30μM Cisplatin treatment for 24hr) , PC-12 , Rat2

Validation: IP/MS, KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur PARP1

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Autre désignation: poly(ADP-ribose) polymerase 1

Sujet: Poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1,This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Poids moléculaire: 113 kDa

ID gène: 142

UniProt: P09874

Pathways: Apoptose, Caspase Cascade in Apoptosis, Réparation de l'ADN, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location