p53 anticorps (AA 14-389)

N° du produit ABIN7464418

L’anticorps anti-p53 Monoclonal Souris est utilisé pour la détection de p53 dans des échantillons de Humain. Il a été validé pour WB, ELISA, IF, FACS, IHC (p), IP, ICC et IHC (fro).

Aperçu rapide pour p53 anticorps (AA 14-389) (ABIN7464418)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Pab240

Détail du produit anti-p53 anticorps

Épitope: AA 14-389

Réactivité croisée: Cat, Humain, Souris

Purification: Protein G purified

Immunogène: Gel-purified p53 containing p53 amino acids 14-389 (derived from the pSV53C p53 cDNA clone).

Isotype: IgG1

Information d'application

Indications d'application: For IHC: Use at a dilution of 1:250 - 1:500. Extensive washing may be necessary. This product does not require protein digestion or microwave antigen retrieval prior to staining. For IP: Use at 10 μg/mg of lysate. By immunoprecipitation, this antibody reacts with only MUTANT p53protein under NON-DENATURING conditions. For WB: Use at a dilution of 1:2,000. Predicted molecular weight: 53 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Commentaires:

Positive Control: HBL100 , HCT116 , 293T , A431

Validation: Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 2 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: tumor protein p53

Sujet: Tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53,This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Poids moléculaire: 44 kDa

ID gène: 7157

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg