ATM anticorps (AA 2577-3056)

N° du produit ABIN7467993

Cet anticorps Souris Monoclonal détecte spécifiquement ATM dans WB, IHC, ELISA, IF, IP, IHC (p), FACS, ICC et ChIP. Il présente une réactivité envers Humain.

Aperçu rapide pour ATM anticorps (AA 2577-3056) (ABIN7467993)

Antigène: ATM (Ataxia Telangiectasia Mutated (ATM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ATM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Classe de qualité: KO Validated

Clone: 2C1

Détail du produit anti-ATM anticorps

Épitope: AA 2577-3056

Réactivité croisée: Humain, Singe, Souris, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.

Isotype: IgG1

Information d'application

Indications d'application: Recommended Starting Dilutions:
For WB: Use at a dilution of 1:500-1:3000. Predicted 350 kDa.
For IHC-P: Use at 5 μg/mL. Antigen retrieval in Citrate buffer is recommended.
For IP: Use at a concentration of 1-10 μg/mL.
For ICC/IF: Please refer to the publication by Harry Scherthan, et.al., 2000 and Yiyoung Liu, et.al., 2006.
For FACS:Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.

Commentaires:

Positive Control: SK-N-SH , HeLa , HeLa nuclear extract Validation: IP/MS, KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur ATM

Antigène: ATM (Ataxia Telangiectasia Mutated (ATM))

Autre désignation: ATM serine/threonine kinase

Sujet:

Synonyms: ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

Background: The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates, thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Poids moléculaire: 351 kDa

ID gène: 472

UniProt: Q13315

Pathways: Signalisation p53, Apoptose, Réparation de l'ADN, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus